Intended Use

This kit is  used to qualitative detection of feline calicivirus (FCV) antigen in feline ocular nasal secretion, and can be used for screening and assisting diagnosis of feline calicivirus infection.

Principle

Feline Calicivirus Antigen Rapid Test Kit was used to qualitative detection feline calicivirus in feline ocular nasal secretion by colloidal gold immunochromatography. The feline calicivirus antibody is coated on the nitrocellulose membrane. If the sample contains feline calicivirus, the virus will bind to the colloidal gold coated feline calicivirus monoclonal antibody on the gold label mat to form an antibody-antigen complex. When this complex was chromatographed to the detection line of the membrane (T line), it was combined with another feline calicivirus monoclonal antibody to form an “antibody-antigen-antibody” sandwich complex and gradually agglutinated into a visible T line. The unconjugated colloidal gold antibody was chromatographed to the T line and was captured by the quality control line (C line) and formed a visible C line. The test results were displayed through the C line and T line. If the sample contains feline calicivirus, the T line shows a red band. If the sample does not contain feline calicivirus, the T line does not show a red band. The C line shows a red color band regardless of whether the sample contains feline calicivirus.